Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EN1

Cell type

Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA

Attributes by original data submitter

Sample

source_name
Dermal fibroblasts
cell type
Human dermal fibroblasts
treatment
TGFbeta
phenotype
knockdown of EN1
chip antibody
EN1 (NBP2-57757, Novus Biologicals)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP assays, including purification of the enriched DNA, were performed using the ChIP-IT High Sensitivity Kit (Active Motif). 20-30 μg of sonificated chromatin extract was incubated with 1 µg of antibodies against EN1 or SP1. Libraries were prepared according to standard Illumina protocols

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
22851209
Reads aligned (%)
98.4
Duplicates removed (%)
14.6
Number of peaks
518 (qval < 1E-05)

hg19

Number of total reads
22851209
Reads aligned (%)
98.0
Duplicates removed (%)
14.7
Number of peaks
372 (qval < 1E-05)

Base call quality data from DBCLS SRA